The second leg of the 1872 Cup between Glasgow Warriors and Edinburgh Rugby will be played on 26 April at Scotstoun.
The date was confirmed as Edinburgh's RaboDirect Pro12 run-in was revealed by the league.
Glasgow won the 1872 Cup first-leg 20-16 at Murrayfield thanks to a Stuart Hogg try.
Edinburgh will play their last home league game of the season against Munster at Meggetland on 3 May.
"We're delighted that our season run-in is now confirmed, with the details of some very important matches now set in stone," said Edinburgh Rugby managing director David Davies.
"The 1872 Cup match against Glasgow Warriors is a huge match for vital league points against our biggest rivals."
Warriors are hoping to beat their previous attendance record of 8,852 for a game in Glasgow when Edinburgh visit, having increased the capacity of Scotstoun to around 10,000.
Edinburgh Rugby RaboDirect PRO12 run-in
Thursday 3 April: Dragons v Edinburgh Rugby, Rodney Parade (18:30 BST, live on BBC Alba)
Friday 11 April: Edinburgh Rugby v Cardiff Blues, Meggetland (19:45 BST)
Saturday 19 April: Zebre v Edinburgh Rugby, Stadio XXV Aprile (14:30 BST, live on BBC Alba)
Saturday 26 April: Glasgow Warriors v Edinburgh Rugby, Scotstoun Stadium (19:05 BST)
Saturday 3 May: Edinburgh Rugby v Munster Rugby, Meggetland (17:00 BST, live on BBC Alba)
Saturday 10 May: Leinster Rugby v Edinburgh Rugby, RDS (18:30 BST)