Three Grenoble players have been charged with gang rape, reports AFP.
Prosecutors said there was "serious or consistent evidence" against France's Loick Jammes, New Zealander Rory Grice and Irishman Denis Coulson.
The trio, who face a maximum sentence of 20 years in jail if they are found guilty, cannot leave France.
Jammes' lawyer said the charges allowed legal teams to "put forward a real defence" of their clients, "respecting the presumption of innocence".
A woman told police she had been drugged and raped in a hotel in Bordeaux following a league match between Bordeaux and Grenoble on March 11.
A lawyer for Coulson denied the allegations, describing the incident as "a night out between consenting adults".
Grenoble had suspended six players but reinstated them, citing a "presumption of innocence".