Victory for the Czech Republic in Tuesday's Euro 2012 qualifying matches moved them five points clear of Scotland in Group I.
Goals from Milan Baros and Michal Kadlec in Prague saw the Czechs beat Liechtenstein 2-0.
The result sees the second-placed Czechs on nine points, although they have played a game more than Scotland.
Spain recorded a 3-1 win in Lithuania to stay top of the group with five wins out of five.
Barcelona's Xavi opened the scoring for the visitors but Marius Stankevicius levelled on the hour mark.
However, an own goal from Tadas Kijankas and a late strike from Juan Mata sealed the points for Spain.
Scotland sit third in the table, with four points from four games following defeats to Spain and the Czechs, a draw with Lithuania and victory over the Faroe Islands.
Lithuania have the same points total but have a goal difference two worse than the Scots.