Czech Republic coach Michal Bilek lamented the absence of Chelsea goalkeeper Petr Cech as he named his squad to face Scotland on 3 September.
Cech suffered a medial ligament strain after a heavy fall in training last week and is expected to be out for three to four weeks.
And he has been left out of the 22-man squad for the Euro 2012 qualifier.
"Petr Cech is a world-class goalkeeper," said Bilek. "His injury is naturally very bad news for us."
Four players from Czech champions Viktoria Pilsen have been included in the squad for the Group I match at hampden and a friendly against Ukraine three days later in Prague as Bilek tries to end a series of poor results.
His side are second in Group I, six points behind world champions Spain but five points ahead of the Scots, who have a game in hand.
However, the Czechs suffered a 3-0 defeat by Norway in Oslo two weeks ago and have won only one of their last six matches.
"With a performance like, that we wouldn't have any chance in Glasgow," admitted Bilek.
Czech Republic squad
Goalkeepers: Jaroslav Drobny (Hamburg), Jan Lastuvka (Dnepropetrovsk), Ales Hruska (Pribram)
Defenders: Zdenek Pospech (Mainz), Jan Rajnoch (Ankaragucu), Tomas Sivok (Besiktas), Theo Gebre Selassie (Slovan Liberec), Roman Hubnik (Berlin), Daniel Pudil (Genk), Petr Jiracek (Viktoria Pilsen), Michal Kadlec (Leverkusen)
Midfielders: Tomas Rosicky (Arsenal), Tomas Hubschman (Shakhtar Donetsk), Daniel Kolar (Viktoria Pilsen), Jaroslav Plasil (Bordeaux), Kamil Vacek (Sparta Prague), Jan Rezek (Famagusta), Vaclav Pilar (Viktoria Pilsen), Milan Petrzela (Viktoria Pilsen)
Strikers: Milan Baros (Galatasaray), David Lafata (Jablonec), Tomas Pekhart (Nuremberg)