Sevilla striker Alvaro Negredo has pulled out of the Spain squad for the upcoming Euro 2012 qualifiers against Czech Republic and Scotland.
Negredo has a hamstring problem but the Spanish football federation say coach Vicente del Bosque will not be calling up a replacement.
Barcelona midfield duo Cesc Fabregas and Andres Iniesta had already been ruled out of the two matches.
World and European champions have already qualified for the finals.
However, the winners of qualifying Group I still have a perfect record to maintain, having won all six fixtures so far.
Spain travel to Prague to take on Czech Republic on Friday before hosting Scotland on Tuesday.
These matches are likely to play a significant role in finishes second in the group and clinches a play-off spot for next year's finals in Ukraine and Poland.
The Czechs are currently second in the group, with a two-point advantage and better head-to-head record over The Scots.
Valencia full-back Jordi Alba is the only new face in del Bosque's pool but the coach hinted that he might tinker with his star-studded squad.
"We want to gather as much information as we can as we prepare for the European Championship so we make as few errors as possible," said the former Real Madrid manager.
Goalkeepers: Iker Casillas (Real Madrid), Victor Valdes (Barcelona), Pepe Reina (Liverpool)
Defenders: Alvaro Arbeloa (Real Madrid), Sergio Ramos (Real Madrid), Raul Albiol (Real Madrid), Gerard Pique (Barcelona), Carles Puyol (Barcelona), Jordi Alba (Valencia)
Midfielders: Xabi Alonso (Real Madrid), Sergio Busquets (Barcelona), Santi Cazorla (Malaga), Thiago Alcantara (Barcelona), Xavi (Barcelona), Javi Martinez (Athletic Bilbao)
Forwards: David Silva (Manchester City), David Villa (Barcelona), Pedro (Barcelona), Fernando Torres (Chelsea), Fernando Llorente (Athletic Bilbao), Juan Mata (Chelsea)