British number two James Ward was unable to repeat his Queen's heroics as he lost out to Janko Tipsarevic in the Aegon International first round.
Having reached the semis and leapt to 176 in the world as a result last week, Ward entered the match on a high.
But resuming on Wednesday tied at one set apiece, Ward lost 3-6 6-4 2-6.
British number one Elena Baltacha went out of the women's side of the draw as she was outgunned by Victoria Azarenka in their second-round match.
Baltacha, who won the Aegon Challenge in Nottingham last week, entered the match on a run of six straight wins on grass.
But Belarusian third seed Azarenka, ranked number five in the world, looked in fine shape as she broke the Scot four times, winning 6-1 7-6 (7-0).
"She was always on me, always," said Baltacha. "She was very solid today mentally in how she was delivering and she made it really difficult. It was a great experience, though.
"I couldn't ask for better preparation, winning last week in Nottingham and playing someone in the world's top five going into Wimbledon.
"I'm very happy right now with everything. I think I'm in great form."
The 27-year-old will now have a break before Wimbledon starts next week, where she hopes to land a favourable draw.
"I'd like a kind draw, but I think everyone says that going into a Grand Slam," she said. "Unfortunately, someone has to get the top seeds. I'm just hoping it's not going to be me.
"If it is me, then I've got to deal with it and I've got to go out there and give it everything I have because if you don't, then what's the point going on court? I'm not afraid of anyone."