British pair Colin Fleming and Ross Hutchins failed to complete a brave comeback as Christopher Kas and Alexander Peya knocked them out of the men's doubles in the quarter-finals.
The first home pair to reach this stage since 1993 came back from two sets down before losing 6-4 6-4 6-7 2-6 6-4.
Fleming, with Jocelyn Rae, later beat Dick Norman and Lisa Raymond 6-4 3-6 6-4 in the mixed doubles second round.
But Britain's Jamie Murray and his partner Jarmila Gajdosova went out.
They lost 7-6 (7-5) 6-2 to sixth-seeded Indian duo Rohan Bopanna and Sania Mirza in the second round.
Murray, brother of Andy, had been hoping to add a second mixed title to the one he won in 2007 with Jelena Jankovic.
Meanwhile, Hutchins and fellow British player Heather Watson were beaten 6-4 6-4 to Nenad Zimonjic and Katarina Srebotnik.
Spain's Feliciano Lopez, whom , also lost his mixed doubles third-round contest with Germany's Andrea Petkovic 6-4 6-4 to Bob Bryan and Liezel Huber.
Earlier, Bryan and his twin brother Mike - the top seeds - battled into the men's doubles quarter-finals while defending champions Jurgen Melzer and Philipp Petzschner also progressed.
The Bryans beat unseeded Simon Aspelin and Paul Hanley 6-3 4-6 6-7 (5-7) 6-3 16-14.
The match resumed on Wednesday tied at 3-3 in the deciding set, having been called off the previous evening because of rain, and when the Bryans eventually triumphed the set had lasted 101 minutes.
On Court 14, defending champions Melzer and Petzschner eventually beat the American duo Scott Lipsky and Rajeev Ram 6-3 7-6 (7-3) 4-6 6-3 in the second round.
They later returned to the same court on Wednesday to clinch their quarter-final place by beating Wesley Moodie and Dick Norman, the 11th seeds, 7-6 (7-5) 6-3 4-6 6-2.
Many of the top seeds have already made an early exit, but sixth seeds Nenad Zimonjic and Michael Llodra went through with a straightforward 6-4 7-6 (11-9) 7-6 (7-4) win over Carsten Ball and Santiago Gonzalez.
In the women's doubles, Germany's singles semi-finalist Sabine Lisicki and partner Sam Stosur beat Sorana Cirstea and Ayumi Morita 6-4 6-3 in the third round while Nadia Petrova and Anastasia Rodionova defeated Vera Dushevina and Ekaterina Makarova 7-6 (7-2) 6-4.
In the other match, a quarter-final, Marina Erakovic and Tamarine Tanasugarn took some time to see off the challenge of Liezel Huber and Lisa Raymond 4-6 7-6 (7-1) 13-11.